Table 2.
Detection of Puumala orthohantavirus (PUUV) RNA by RT-PCR, anti-PUUV IgG antibodies by ELISA and RNAscope in situ hybridization (ISH)-based detection of positive-strand RNA in bank voles from the breeding facility (BF).
| No. of Positive/Total Number of Investigated Voles | |||
|
RT-PCR Pos
ELISA Pos (Persistent Infection) |
RT-PCR Neg
ELISA Pos |
||
| BF | BF | ||
| 12/13 | 1/13 | ||
| Detection of Viral Positive-Strand RNA by ISH | |||
| Total | 3/4 (75%) | 1/1 (100%) | |
| Cerebrum (neurons, glia cells, endothelial cells, plexus choroideus [cell with lancet-shaped nucleus in the pia mater]) |
1/3 (33%) | 0/1 (0%) | |
| Cerebellum (Stratum moleculare, -ganglionare and -granulosum) | 1/3 (33%) | 0/1 (0%) | |
| Lung | 3/3 (100%) | 1/1 (100%) | |
| (a) Bronchiolar epithelial cells | 1/3 (33%) | 0/1 (0%) | |
| (b) Pneumocytes type I and II (c) Endothelial cells |
3/3 (100%) | 1/1 (100%) | |
| 1/3 (33%) | 0/1 (0%) | ||
| (d) IC with spindle-shaped and round-oval nuclei | 3/3 (100%) | 1/1 (100%) | |
| Glandula submandibularis (acini) | 1/2 (50%) | n.i. | |
| Glandula mandibularis (acini) | 1/1 (100%) | n.i. | |
| Glandula parotidea (acini) | 1/1 (100%) | n.i. | |
| Tongue (myocytes, IC with lancet to spindle-shaped nuclei) | 1/1 (100%) | n.i. | |
| Liver (Kupffer cells) | 2/3 (66%) | 0/1 | |
| Pancreas (a) Acini (b) Islet cells of Langerhans (c) IC with spindle-shaped and round-oval nuclei |
3/3 (100%) | n.i. | |
| 3/3 (100%) | n.a. | ||
| 1/3 (33%) | n.a. | ||
| 2/3 (66%) | n.a. | ||
| Stomach/Pars non-glandularis (epithelial) | 2/2 (100%) | 0/1 (0%) | |
|
Stomach/Pars glandularis (IC with lancet to spindle-shaped and round-oval nuclei) |
2/2 (100%) | 0/1 (0%) | |
| Duodenum | IC with spindle-shaped nuclei | 3/3 (100%) | 0/1 (0%) |
| Caecum | 1/1 (100%) | n.i. | |
| Colon ascendens | 1/1 (100%) | n.i. | |
| Colon descendens | 2/3 (66%) | 0/1 | |
|
Kidney (a) Glomerulum cells * (b) IC with spindle-shaped nuclei (cortex) |
3/3 (100%) | 1/1 (100%) | |
| 3/3 (100%) | 1/1 (100%) | ||
| 2/3 (66%) | 0/1 (0%) | ||
| Heart (kardiomyocytes) | 2/3 (66%) | 1/1 (100%) | |
|
Adrenal gland (endocrine cells, IC with lancet to plump, spindle-shaped nuclei (cortex and medulla)) |
1/1 (100%) | n.i. | |
| Brown adipose tissue (interstitium) ** | 1/1 (100%) | n.i. | |
No PUUV positive-strand RNA was detected in the negative control bank vole. IC, interstitial cells; *, glomerulum cells include endothelial cells, mesangial cells and podocytes; **, affected cell type not identifiable; n.a., not applicable; n.i., not investigated.