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. 2023 Mar 19;13(6):1102. doi: 10.3390/nano13061102

Figure 2.

Figure 2

Uptake pathway of layered double hydroxides (LDHs) into cells. (a) A representative process of clathrin-mediated endocytosis (b) Confocal microscopy, which includes the colocalization of fluorescein 5′-isothiocyanate (FITC)-LDH and clathrin in MNNG/HOS cells (involving the localization of (1) the nucleus, (2) clathrin, and (3) FITC-LDH in MNNG/HOS cells, as shown in (4) the merged image). Cells were incubated with FITC-LDH for 2 h, treated with clathrin antibodies, and stained by Texas Red and 4′,6-diamidino-2-phenylindole. Scale bar = 10 µm. (Reprinted with permission from Ref. [31], Copyright 2006, the American Chemical Society.).