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. 2023 Mar 1;12(3):391. doi: 10.3390/pathogens12030391

Table 1.

Primers for characterization of CVBPs in pet animals.

Pathogen Target Genes Primer Sequences (5′–3′) Amplicon Size (bp) References
Babesia 18S rRNA GTTTCTGMCCCATCAG
CTGTATTGTTATTTCTTGTCACTACCTC
422–440 [21]
Anaplasma groEL AAGGCGAAAGAAGCAGTCTTA
CATAGTCTGAAGTGGAGGAC
724 [22]
Ehrlichia gltA TTATCTGTTTATGTTATATAAGC
CAGTACCTATGCATATCAATCC
1251 [23]
Hepatozoon 18S rRNA ATACATGAGCAAAATCTCAAC
CTTATTATTCCATGCTGCAG
666 [24]
Bartonella gltA GGGGACCAGCTCATGGTGG
AATGCAAAAAGAACAGTAACA
379 [25]
Mycoplasma 16S rRNA ATACGGCCCATATTCCTACG
TGCTCCACCACTTGTTCA
595–618 [26]
Rickettsia ompB CGACGTTAACG GTTTCTCATTCT
ACCGGTTTCTTTGT AGTTTTCGTC
252 [27]

groESL, heat-shock protein gene; gltA, citrate synthase gene; ompB, outer-membrane protein B.