Table 1.
Pathogen | Target Genes | Primer Sequences (5′–3′) | Amplicon Size (bp) | References |
---|---|---|---|---|
Babesia | 18S rRNA | GTTTCTGMCCCATCAG CTGTATTGTTATTTCTTGTCACTACCTC |
422–440 | [21] |
Anaplasma | groEL | AAGGCGAAAGAAGCAGTCTTA CATAGTCTGAAGTGGAGGAC |
724 | [22] |
Ehrlichia | gltA | TTATCTGTTTATGTTATATAAGC CAGTACCTATGCATATCAATCC |
1251 | [23] |
Hepatozoon | 18S rRNA | ATACATGAGCAAAATCTCAAC CTTATTATTCCATGCTGCAG |
666 | [24] |
Bartonella | gltA | GGGGACCAGCTCATGGTGG AATGCAAAAAGAACAGTAACA |
379 | [25] |
Mycoplasma | 16S rRNA | ATACGGCCCATATTCCTACG TGCTCCACCACTTGTTCA |
595–618 | [26] |
Rickettsia | ompB | CGACGTTAACG GTTTCTCATTCT ACCGGTTTCTTTGT AGTTTTCGTC |
252 | [27] |
groESL, heat-shock protein gene; gltA, citrate synthase gene; ompB, outer-membrane protein B.