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. 2023 Feb 22;21(3):137. doi: 10.3390/md21030137

Figure 6.

Figure 6

N–5 promoted Nrf2 nuclear translocation and upregulated antioxidant enzyme expressions in the ARPE-19 cell model of acrolein damage. The cells were pretreated with 400 μM N–5 for 48 h and then treated with 75 μM acrolein for an additional 24 h, and mRNA and protein levels were analyzed. Western blot image of nuclear Nrf2 (A) and quantification of Western blots (B), mRNA expression of HO-1 (C) and NQO1 (D). The data expressed as ratio relative to controls. Values are mean ± SD of three separate experiments. ## p < 0.01 vs. control (no acrolein, no N–5); ** p < 0.01 vs. acrolein.