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. 2023 Feb 21;11(3):495. doi: 10.3390/vaccines11030495

Figure 5.

Figure 5

The effects of AST VII derivatives (DC-AST VII and DAC-AST VII) on the maturation and activation of BMDCs in the absence/presence of LPS. BMDCs generated from C57BL/6 mice bone marrow cells were treated with DC-AST VII or DAC-AST VII at the concentrations of 2, 5, 10, or 20 µM for 24 h. The expression of (A) MHCII, (B) CD86, and (C) CD80 on CD11c+MHCII+ BMDCs without LPS treatment and (D) MHCII, (E) CD86, and (F) CD80 on CD11c+MHCII+ BMDCs with LPS treatment was determined using flow cytometry. Representative histograms (top panel) and summary graphs (bottom panel) are shown. Data are shown as the mean and SD of triplicates and are representative of two independent experiments with similar results. Statistically significant differences between the treated group were analyzed compared to the control (untreated cells). Intragroup and intergroup comparisons were performed using one-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. (not statistically significant).