Table 2.
Identification of Codon 250 as a Mediator of Isoform and Cross-species Selectivity
| Species ↓ residue → | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 253 |
|---|---|---|---|---|---|---|---|---|---|
| CTPS1_Hs | D | V | S | S | I | Y | R | V | P |
| CTPS1_Cf | D | V | S | S | I | Y | R | V | P |
| CTPS1_Rn | D | V | S | S | I | Y | R | V | P |
| CTPS1_Mm | D | V | S | S | I | Y | R | V | P |
| CTPS2_Hs | D | V | S | S | T | Y | R | V | P |
| CTPS2_Cf | D | V | S | S | T | Y | R | V | P |
| CTPS2_Rn | D | V | S | S | I | Y | R | V | P |
| CTPS2_Mm | D | V | S | S | I | Y | R | V | P |
The table shows amino acid residues for positions around residue 250 which is predicted to project into the ATP binding pocket of the CTPS enzyme; alignment of CTPS1 and CTPS2 sequence across different species is shown.
Cf = Canis familiaris (dog); CTPS = cytidine triphosphate synthase; Hs = Homo sapiens (human); Mm = Mus musculus (mouse); Rn = Rattus norvegicus (rat).