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Comparison of single-cell and bulk MS2. For the peptide GFAFVTFDDHDSVDK, the top spectrum is from single-cell data, and the bottom spectrum is from bulk data. The bulk spectrum has 17 annotated y-ions and single-cell only has five annotated y-ions. A similar fraction of b-ions are lost in single cell spectra. The lost peaks are mostly from medium and low intensity peaks.
