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. 2023 Mar 23;27:1–14. doi: 10.1016/j.bioactmat.2023.03.007

Fig. 7.

Fig. 7

Exosome-encapsulated miR-25-3p counteracts ferroptotic injury in response to ICH via regulating p53/SLC7A11/GPX4 pathway.

(A–B) Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression levels of SLC7A11 and GPX4 around hematoma from different groups (n = 3 animals for each group). (C) Representative Western blotting bands showing the level of SLC7A11 and GPX4 around hematoma from different groups. β-actin and β-Tubulin was served as a loading control. (D–E) Quantitative analysis of GPX4 and SLC7A11 expression level. (F) Representative Western blot of P53 around hematoma from different groups. GAPDH was served as a loading control. (G) Quantitative analysis for the level of P53. n = 6 animals for each group, *p < 0.05, **p < 0.01, ***p < 0.001 for ICH vs. sham; #p < 0.05, ##p < 0.01, for ICH + EXO or ICH + miR-25-3p vs. ICH. (H–I) Luciferase reporter assay demonstrating direct interaction of miR-25-3p with 3′UTR of the P53 gene. One-way ANOVA and Tukey's post hoc test, n = 3, **P < 0.01.