Fig. 4. Tum1 oxidizes roGFP2 independently of forced proximity.
a, Response of cytosolic (ct) Tum1-roGFP2 to l-Cys in the parental (WT, left) and Δtum1 strain (right). b, In vitro response of roGFP2 (100 nM) to increasing 3MP concentrations in the presence of an equimolar amount of 100 nM wild-type (left panel) or mutant Tum1 (right panel). c, In vitro response of the Tum1-roGFP2 fusion protein (100 nM) to 3MP (solid lines) in comparison to the response of an equimolar mixture of 100 nM roGFP2 and 100 nM Tum1 (dashed lines). d, In vitro response of roGFP2 (100 nM) to 3MP in the presence of increasing concentrations of Tum1. All data are based on n = 3 independent experiments.