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. Author manuscript; available in PMC: 2023 Mar 30.
Published in final edited form as: Nature. 2022 Oct 26;611(7935):387–398. doi: 10.1038/s41586-022-05365-x

Extended Data Fig. 12. Effect of 5-AZA treatment on DNA methylation and cell phenotypes.

Extended Data Fig. 12.

a, CCK-8 assay started with n=3 biological replicates of 1,000 cells per well in 96-well plate. The assay completed on day 8 as the DMSO group grow confluent. Data show mean ± s.e.m. MW: molecular weight. b, Western Blot results for Caspase-3. AA2 is Apoptosis Activator 2 (TOCRIS 2098). The experiments were independently repeated twice with similar results. c, Western Blot for yH2AX. UV light condition is detailed in method. The experiments were independently repeated twice with similar results. d, Flow cytometry result for cells treated with either DMSO or 5-AZA 0.5μM. e, Flow cytometry results for cells treated with 1μM, 2μM, 4μM, and 8μM 5-AZA for 2 days.