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. 2023 Mar 29;13:5153. doi: 10.1038/s41598-023-32276-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The risk signature is associated with immunophenotypes (A) Screened out BTK and DPEP2 and constructed risk model by LASSO analysis with optimal lambda values (B) K-M survival analysis of patients in high- and low-risk groups. (C) The LUAD patients’ risk score distribution and survival status distribution. (D) Univariate and multivariate Cox regression analyses of the association between the prognosis of patients and different clinical-pathological factors(After exclusion of missing values, a total of 336 patients were included in the analysis). (E) Construction of a nomogram. (F) The construction of the calibration curve for the nomogram model, including three colored lines (blue, red, and purple) represent the performance of the nomogram. A closer fit to the diagonal gray line indicates a better estimation. (G) Distribution of risk score in two immune subtypes of LUAD patients. (H) Correlation of risk score with patients' immune status using different immune scoring approaches to assess risk score (***p < 0.001; **p < 0.01; *p < 0.05).