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. 2023 Mar 29;14:1753. doi: 10.1038/s41467-023-37408-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Immunofluorescence staining on spread meiotic spermatocyte preparations from wild-type C57Bl/6 mice using antibodies against SYCP3 (green) and Pol II (a), Ser5P (b), and Ser2P (c) (magenta). Leptonema (L), zygonema (Z), pachynema (P), diplonema (D), and diakinesis (Dia) staged spermatocytes are defined by SYCP3 morphology. The X and Y chromosomes are outlined in white. Arb. units = arbitrary units. d–f Quantification of signal intensity for Pol II, Ser5P, and Ser2P immunofluorescence staining in wild-type spermatocytes. Average intensity is shown, and error bars represent 95% confidence intervals. Samples were obtained from three 3-month-old mice. Source data are provided as a Source data file. d n = 606 prophase I cells. e There was no significant difference in the fluorescence intensity of Ser5P between prophase I substages. n = 586 prophase I cells. f n = 677 prophase I cells. g Radioactive nuclear run-on results comparing the relative levels of nascent transcription between leptonema/zygonema (LZ), pachynema (P), diplonema (D), and round spermatids (RS). Average relative transcriptional activity is shown, and error bars represent standard deviation. Source data are provided as a Source data file. d–g *p-value = 0.012, **p-value = 0.0056, ****p-value <0.0001, one-way ANOVA with the post hoc Tukey’s multiple comparison’s test.