Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 29;14:1753. doi: 10.1038/s41467-023-37408-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Schematic of experimental design for inhibition of BRDT with the small molecule, JQ1. 7-week-old male mice were injected intraperitoneally (i.p.) with JQ1 or vehicle solution daily for 3 weeks. Created with BioRender.com. b, c Immunolocalization of SYCP3 (green) and Ser5P (b) and Ser2P (c) (magenta) in pachytene (top) and diplotene (bottom) spermatocytes from JQ1-treated and vehicle-treated male mice. The X and Y chromosomes are enclosed within white dotted circles. d, e Quantification of signal intensity for Ser5P and Ser2P immunofluorescence staining of pachytene (left) and diplotene (right) cells from JQ1 and vehicle-treated male mice. Average intensity is shown, and error bars represent the standard deviation. Samples were obtained from three biological replicates. Arb. units = arbitrary units. Source data are provided as a Source data file. d n = 66 vehicle-treated and 160 JQ1-treated pachytene spermatocytes; n = 29 vehicle-treated and 80 JQ1-treated diplotene spermatocytes. e n = 34 vehicle-treated and 148 JQ1-treated pachytene spermatocytes; n = 19 control and 66 JQ1-treated diplotene spermatocytes. ****p-value <0.0001, Mann–Whitney U test.