Fig. 3.

IFNγ secretion from IL-1RAP-CAR T-cells after stimulation with rIL-1RAP. A Quantification of IFNγ secretion in the supernatant of untransduced T-cells and IL-1RAP-CAR T-cells co-cultured with different concentrations of coated rIL-1RAP. Data are presented as mean ± SD for 3 independent experiments from 3 different donors. B Concentration of IFNγ (pg/ml) in the supernatant of untransduced T-cells and IL-1RAP-CAR T-cells co-cultured at an E:T ratio of 1:5 for 6 h with target cells expressing or not expressing rIL-1RAP (Mono-Mac-6 and K562, respectively) and co-cultured with coated rIL-1RAP. Data are presented as mean ± SD for 3 independent experiments. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05