Fig. 2.

Control panel of the user interface of VIsoQLR. For visualization purposes, the control panel shown in the application as a single column is here split into six subpanels. a Input subpanel where users can select the input file format and upload the aligned sequences. b Analysis bounding subpanel allows the analysis of the gene and sequence area. It also contains an option to analyze only the full-length PCR sequences. c Exon coordinates subpanel, with the option to automatically detect consensus exon coordinate (CEC). d External isoforms subpanel, where users can upload known or previously defined transcripts as a reference to curate the isoforms detected by VIsoQLR. e Display options subpanel. Here the user can filter the isoforms to be displayed based on their abundance and fine-tune the graphics. f Download prefix subpanel is used to indicate the prefix of all downloadable tables and figures