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. 2023 Mar 30;6:341. doi: 10.1038/s42003-023-04701-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Visible fluorescence of PpIX illuminated under blue light. b PpIX concentration map generated from hyperspectral data acquired with the LCTF. c PpIX concentration map generated with IRIS using channels from the 1^st polarization. d PpIX concentration map generated with IRIS using channels from the 2^nd polarization. Concentration maps are overlaid on RGB images of the surgical field of view acquired with the Pentero operating microscope. Green box represents the field of view of the hyperspectral imaging system used to detect fluorescence. Color scale bars show imaged PpIX concentrations in micrograms per milliliter (μg/mL). Spatial scale bar (1 cm) appears in a).