Table 7.
Purification table for β-glucosidase from Neofusicoccum parvum strain F7.
| Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) | Fold purity | |
|---|---|---|---|---|---|
| Crude extract | 90 | 1042.30 | 11.58 | 100 | 1 |
| Ammonium sulphate fraction | |||||
| Combined 80%, 90%, 100% | 31.90 | 288.70 | 9.05 | 27.7 | 2.20 |
| Supernatant | 40 | 5 | 0.13 | 0.48 | 0.01 |
| Anion exchange (HiTrap Q FF) | |||||
| Combined ammonium sulphate fraction | 4.20 | 125.13 | 29.79 | 12.01 | 2.57 |
| Gel extraction | |||||
| Bgl1 | 1 | 21.80 | 21.8 | 2.09 | 2.42 |
| Bgl2 | 0.60 | 10.80 | 18 | 1.04 | 4.33 |
| Bgl3 | 0.20 | 76.80 | 384 | 7.37 | 33.22 |