TABLE 1.
Statistical analysis of the blood concentration of the different baclofen enantiomers and their metabolites after an i.p. injection of a dose of 1.5 mg/kg at 30 and 240 min post-administration. Statistical analysis of the blood concentrations of the different forms of baclofen and their metabolites. The enantiomers and the racemic form were i.p. injected at a dose of 1.5 mg/kg in males and females, then blood was collected 30 and 240 min after the injection. Samples were analyzed by HPLC. Bold characters indicate a significant main effect and/or an interaction following a 2-way RM ANOVA.
| Figure number | Factor name | F values | p-value |
|---|---|---|---|
| Figure 4A: Blood RS-Baclofen concentration in males and females | Sex | F(1,13) = 3.82 | p = 0.073 |
| 2-way RM ANOVA | Timepoint | F(1,13) = 105.89 | p < 0.001 |
| Sex x Timepoint | F(1,13) = 4.23 | p = 0.060 | |
| Figure 4B: Blood Metabolite of RS-Baclofen concentration in males and females | Sex | F(1,13) = 1.70 | p = 0.215 |
| 2-way RM ANOVA | Timepoint | F(1,13) = 67.54 | p < 0.001 |
| Sex x Timepoint | F(1,13) = 3.38 | p = 0.086 | |
| Figure 4C: Ratio Metabolites/RS-Baclofen blood concentration in males and females | Sex | F(1,26) = 2.161 | p = 0.154 |
| 2-way RM ANOVA | Timepoint | F(1,26) = 13.304 | p < 0.001 |
| Sex x Timepoint | F(1,26) = 0.907 | p = 0.35 | |
| Figure 4D: Blood R-Baclofen concentration in males and females | Sex | F(1,12) = 6.37 | p < 0.05 |
| 2-way RM ANOVA | Timepoint | F(1,12) = 208.35 | p < 0.001 |
| Sex x Timepoint | F(1,12) = 6.62 | p < 0.05 | |
| Figure 4E: Blood Metabolite of R-Baclofen concentration in males and females | Sex | F(1,15) = 1.26 | p = 0.28 |
| 2-way RM ANOVA | Timepoint | F(1,15) = 16.99 | p < 0.001 |
| Sex x Timepoint | F(1,15) = 0.67 | p = 0.427 | |
| Figure 4F: Ratio Metabolites/R-Baclofen blood concentration in males and females | Sex | F(1,30) = 1.579 | p = 0.219 |
| 2-way RM ANOVA | Timepoint | F(1,30) = 4.834 | p < 0.05 |
| Sex x Timepoint | F(1,30) = 0.076 | p = 0.785 | |
| Figure 4G: Blood S-Baclofen concentration in males and females | Sex | F(1,13) = 7.706 | p < 0.05 |
| 2-way RM ANOVA | Timepoint | F(1,13) = 233.36 | p < 0.001 |
| Sex x Timepoint | F(1,13) = 0.149 | p = 0.706 | |
| Figure 4H: Blood Metabolite of S-Baclofen concentration in males and females | Sex | F(1,13) = 6.37 | p < 0.05 |
| 2-way RM ANOVA | Timepoint | F(1,13) = 59.47 | p < 0.001 |
| Sex x Timepoint | F(1,13) = 0.213 | p = 0.652 | |
| Figure 4I: Ratio Metabolites/S-Baclofen blood concentration in males and females | Sex | F(1,26) = 10.104 | p < 0.01 |
| 2-way RM ANOVA | Timepoint | F(1,26) = 128.23 | p < 0.001 |
| Sex x Timepoint | F(1,26) = 12.784 | p < 0.001 | |
| Blood concentrations of RS-Baclofen after a dose of 3 mg/kg in males and females | Sex | F(1,12) = 0.090 | p = 0.77 |
| 2-way RM ANOVA | Timepoint | F(1,12) = 1041.80 | p < 0.001 |
| Sex x Timepoint | F(1,12) = 0.038 | p = 0.849 |