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. 2023 Mar 16;11:1152207. doi: 10.3389/fbioe.2023.1152207

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Copyright © 2023 Tilotta, Vadalà, Ambrosio, Cicione, Di Giacomo, Russo, Papalia and Denaro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BM-MSC-derived secretome enhanced hNPC metabolic activity. MTT assay on hNPCs treated with BM-MSC-derived CM (A) or ADSC-derived CM (B) at a 20%, 40%, 80% or 100% concentration (percentage of CM diluted in DMEM, volume/volume) showed that 20% BM-MSC CM yielded the greatest effect towards cell metabolic activity. Data are expressed as percent variation between the control and experimental groups. (C) 20% BM-MSC-derived CM determined a significantly higher increase of metabolic activity in hNPCs compared to 20% ADSC-CM. **p < 0.01, ***p < 0.001, ****p < 0.0001.