Figure 2.

Whole-brain imaging reveals the topographical distribution of Sumo paralogs

(A) Schematic of brain clearing and light sheet microscopy for whole brain imaging with a 3D Imaris render of a His₆-HA-Sumo2 brain.

(B) Heatmaps of anti-HA immunosignal intensity normalized to regional density quantified by NeuN staining and alignment to the Allen Brain Atlas across the His₆-HA-Sumo2 depicted brain region, averaged between hemibrains and biological replicates (N = 2, two hemispheres/replicate).

(C) Bar plot depicting the relative levels of anti-HA immunosignal per voxel averaged across His₆-HA-Sumo2 brain regions defined by the Allen Brain Atlas.

(D) Heatmaps of anti-HA immunosignal intensity normalized to regional density quantified by NeuN staining and alignment to the Allen Brain Atlas across the His₆-HA-Sumo1 depicted brain region, averaged between hemibrains and biological replicates (N = 2, two hemispheres/replicate).

(E) Bar plot depicting the relative levels of anti-HA immunosignal intensity normalized to regional density across His₆-HA-Sumo1 brain regions defined by the Allen Brain Atlas. Each datapoint in (C) and (D) is from the mean intensity from a single hemisphere (N = 2, two hemispheres/replicate). Data are presented as mean ± SEM. See also Figures S2, S3, Table S1, Video S1, and Video S2.