Extranuclear localization of Sumo2 in neurons of the hippocampal CA3 region
(A) Anti-HA (purple), DAPI (blue), MAP2 (red), and anti-Shank2 (green) immunostaining of wild-type (left) and HA-Sumo2 KI (right) hippocampal CA3 region. Scale bar: 50 μm.
(B) Anti-HA signal in masked regions for each marker (exemplified for Shank2 in the bottom panel A) was quantified in both wild-type (WT) and HA-Sumo2 KI, and average intensity was calculated. N = 3, ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0,0005, ∗∗∗∗p < 0,0001. See also Figure S4.