Figure 5.

Neuronal Sumo2 has shared and distinct substrates compared to Sumo1 in vivo

(A) Schematic of approach to identify His₆-HA-Sumo interactors from the adult mouse brain via anti-HA affinity immunoprecipitation and mass spectrometry (N = 4).

(B) Heatmap depicting the relative peptide abundance of His₆-HA-Sumo2 interactors relative to levels in His₆-HA-Sumo1 immunoprecipitation.

(C) gProfiler2 Gene Ontology analysis for His₆-HA-Sumo2 interactors.

(D) Anti-HA affinity immunoprecipitation followed by Western Blot analysis of His₆-HA-Sumo1 and His₆-HA-Sumo2 interactors as listed on the left side (N = 3–6). Black arrowheads indicate protein SUMOylation inferred by shift in molecular weight. See also Figure S6 and Table S2.