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Sequences of real-time PCR primers.
| Gene name | Primers (5′-3′) | Products | GenBank |
|---|---|---|---|
| Claudin-1 | F: TGGCCACGTCATGGTATGG | 62 | NM_001013611 |
| R: AACGGGTGTGAAAGGGTCATAG | |||
| Occluding | F: GAGCCCAGACTACCAAAGCAA | 68 | NM_205128 |
| R: GCTTGATGTGGAAGAGCTTGTTG | |||
| Muc-2 | F: GCCTGCCCAGGAAATCAAG | 59 | NM_001318434 |
| R: CGACAAGTTTGCTGGCACAT | |||
| β -actin | F: GAGAAATTGTGCGTGACATCA | 152 | NM_205518 |
| R: CCTGAACCTCTCATTGCCA |
Muc-2: Mucin-2.
