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Tonbo Fixation/Permeabilization Working Solution (“Fix/Perm”)
| Reagent | Final concentration | Amount |
|---|---|---|
| Tonbo Fix/Perm Diluent (1×) | 3 mL | |
| Tonbo Fix/Perm Concentrate (4×) | 25% | 1 mL |
| Total | 4 mL |
Make fresh day of experiment with appropriate volume required for flow cytometry samples, store on ice or at 4°C.
