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. 2023 Mar 16;10:1070734. doi: 10.3389/fmed.2023.1070734

Figure 5.

Figure 5

TLR4 expression is essential for induction of fut2 mRNA expression and cell surface fucosylation by UEA1 and LPS. To test whether TLR4 expression is sine qua non for Hel cell induction of fut2 mRNA, the consequence of TLR4 knock-down by siRNA on response to the TLR4 ligands was measured (A). The induction of FUT2 mRNA transcription by LPS (right) or by UEA1 (left) is inhibited by a TLR4 specific siRNA, but not by a scrambled siRNA. In contrast, only induction of IL-8 transcription by LPS was affected by the TLR4 siRNA, as the UEA1 did not induce IL-8. The induced cell surface fucosylation, measured by FACS analysis, followed the same pattern (B), consistent with TLR4 mediation of FUT2 mRNA induction regulating cell surface fucosylation; CD49 was the constitutive control. Thus, activation of fucosylated TLR-4 is necessary for inducing fut2 mRNA and cellular fucosylation. Data are mean ± SEM n = 4 (*p < 0.01; **p < 0.001).