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. 2023 Mar 30;8(2):283–298. doi: 10.1089/can.2022.0011

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© Christopher D. Drummond-Main et al. 2023; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2. — CBD reduces the threshold for mPT and calcium uptake in acutely isolated rat brain mitochondria. (A) Representative Ca²⁺ infusion traces demonstrating that CBD, at concentrations >1.875 μM, reduced the threshold for Ca²⁺-induced mPT in acutely isolated rat brain mitochondria. The y-axis of the graph is expressed in CaG5N fluorescence AU. (B) Summary bar graph reflecting the mean±SEM of mPT thresholds at different CBD concentrations. The y-axis indicates the amount of Ca²⁺ added to the mitochondrial suspension. mPT threshold and calcium uptake were significantly reduced by CBD at concentrations >1.875 μM. (C) Shows the concentration–response curve for inhibition of calcium uptake by CBD. CBD, at concentrations lower than ∼2 μM, does not affect mitochondrial mPT and calcium uptake. Data are expressed as mean±SEM (n=3∼8 rats per group) and NS, **p<0.01, and ****p<0.0001; statistical analysis was performed using one-way ANOVA with Tukey post hoc test, [F(8, 41)=(73.42), p<0.0001]. AU, arbitrary units; mPT, mitochondrial permeability transition; NS, nonsignificant.