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. 2023 Mar 30;8(2):283–298. doi: 10.1089/can.2022.0011

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© Christopher D. Drummond-Main et al. 2023; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — CBD potently blocks a novel mitochondrial inner membrane chloride channel. (A) Top graph is a representative mitoplast-attached recording at +30 mV, showing activity of three channels, each with a conductance of ∼100 pS. Addition of 6.4 μM CBD to the bath abolished channel activity. Bottom graph shows that channel activity was partially restored by washout. C=closed state (n=3 per group). (B) The concentration–response curve for the effect of CBD on the open probability (NP open) of a mitochondrial inner membrane chloride channel from acutely isolated Wistar rat brain mitochondria. At low micromolar concentrations, CBD potently inhibited the chloride channel with an IC₅₀ of ∼1.9 μM. Data are pooled from 31 mitoplast recordings and expressed as mean±SEM. (C) The endogenous cannabinoid AEA does not affect mitochondrial chloride channel activity. Chloride channel activity before and after AEA. Top graph is baseline channel activity at +30 mV. Bottom graph is channel activity after bath application of AEA (30 nM; +30 mV; n=3 per group). AEA, anandamide.