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. 2023 Jan 31;14:1091040. doi: 10.3389/fendo.2023.1091040

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Copyright © 2023 Cao, Tan, Yan, Shen, Hua, Shao and Yao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HFD feeding induced macrophage infiltrations in the colon are not affected by SG. (A) Gene expressions of macrophage-related chemokines. (B) Gene expressions of macrophage-related markers. (C) Representative histological images of colon stained with anti-CD68 antibody (400x; scale bar, 50 um) and quantification of CD68 positive cells. (D) Representative FACS analysis of F4/80⁺CD11b⁺CD11c^- cells in colonic lamina propria. The right panel indicates the percentage of F4/80⁺CD11b⁺CD11c^- cells among F4/80+ cells in colonic lamina propria. n = 7–9/group; Data are presented as means ± SEM. Student’s t-test was used for significance assessments. *P < 0.05. SHAM, sham surgery; SG, sleeve gastrectomy.