Fig. 3.

circACTR2 over-expression attenuates GEM-Resistance of PC cells in vitro

A The relative expression levels of circACTR2 in PC cells stably transfected with circACTR2 over-expression vector (OE-circ) and corresponding negative control (NC) vector were detected by qRT-PCR. B Relative cell viability of PC cell transfected with OE-circ vector and OE-NC vector under GEM treatment for 72 h detected by CCK-8 assay. C Apoptosis rate and D cell cycle distribution in PC cells transfected with OE-circ vector and OE-NC vector detected by flow cytometry. E circACTR2 expression in PC cells stably transfected with sh-circACTR2 (sh-circ) vector and sh-NC vector were detected by qRT-PCR. F Relative cell viability of PC cell transfected with sh-circ vector and sh-NC vector under GEM treatment for 72 h. G Apoptosis rate and H cell cycle distribution in PC cells transfected with sh-circ vector and sh-NC vector detected by flow cytometry. *p < 0.05 and **p < 0.01