Fig. 5.

circACTR2 reverse GEM resistance by inhibiting PI3K/AKT pathway via miR-221-3p/PTEN axis

A Schematic graph illustrated the mutation of potential binding site between miR-221-3p and the 3’-UTR regions of PTEN. B The relative luciferase activities of wild type PTEN 3’UTR and its mutant after transfected with miR-221-3p mimic in SW1990 cells. C and D PETN expression in PC cells after over-expressing and silencing miR-221-3p were detected by qRT-PCR, respectively. E and F PETN expression in PC cells after over-expressing and silencing miR-221-3p were detected by Western blotting, respectively. G Pearson correlation analysis of circACTR2 and PTEN expression in 25 PC tissues. H and I Western blotting for PTEN, AKT and p-AKT in PC cell lines transfected with indicated vectors, miR mimic or inhibitors. J and K Relative cell viability of PC cells were determined after treated with sh-PTEN transfection or PTEN OE-vector/LY294002 by CCK-8 assays, respectively. *P < 0.05; **P < 0.01