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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: FASEB J. 2022 Sep;36(9):e22482. doi: 10.1096/fj.202101398R

FIGURE 5.

FIGURE 5

Expression of cyclin genes is significantly reduced in BHLivDKO mice compared to H4LivKO mice. (A) qPCR analysis shows expression of cyclin genes Ccnd1, Ccnb1 and Ccna2 10 days post-tamoxifen injection. Two-way ANOVA, Sidak’s multiple comparisons test (N = 6–8): *p < .03; **p < .005; ***p < .0005, ****p < .0001. (B) qPCR analysis of cyclin genes after DEN and HFD treatment, Two-way ANOVA, Sidak’s multiple comparisons test (N = 8–12): *p < .03; ***p < .0005. (C) Cyclin A2 (Ccna2) expression in Aml12 cells following knockdown of Bmal1, Hnf4α, or both Bmal1 and Hnf4α using siRNA at 0,16, 20, and 40 h post-serum shock. Scrambled oligonucleotide (Sc) was used as a control. Two-way ANOVA, Sidak’s multiple comparisons test (N = 10): *p < .03; **p < .005; ***p < .0005, ****p < .0001. (D) Survival curves show CCNA2, CCNB1, CCND1 genes expression using the tumor liver hepatocellular carcinoma (TCGA) LIHC dataset using the R2 Genomics Analysis and Visualization Platform (http://r2.amc.nl). Survival time measured from the time of initial diagnosis to the date of death or the date of last follow up. The survival distribution estimated by Kaplan–Meier method. p-values <.05 were considered to be statistically significant.