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vPK undergoes ubiquitination. Myc tagged-vPK or STING and HA-ubiquitin expression plasmids were transfected into HEK293T cells in the presence or absence of proteasome inhibitor, MG-132. Forty-eight hours posttransfection, Myc-tagged vPK or STING was immunoprecipitated and the immunoprecipitate was subjected to immunoblotting with the indicated antibodies. The experiments represent at least 3 biological replicates.
