FIG 10.

The 5′ UTR and 3′ UTR of PRV genome DNA were sufficient for caspase-1 activation and ASC oligomerization but not pro-IL-1β transcription. (A to F) The 5′-UTR and 3′-UTR transcripts of PRV genomic DNA were amplified and transfected into mouse peritoneal macrophages isolated from C57BL/6 mice for 24 h. The mRNA levels of IL-1β (A) and IL-18 (B) were measured by qPCR, and the secretion levels of IL-1β were measured by ELISA (C). The protein levels of pro-caspase-1, cleaved caspase-1, and active caspase-1 (p10) in cell lysates were analyzed by Western blotting (D). (E, F) ASC oligomerization was examined with anti-ASC mAb under confocal microscopy. The scale bars represent 10 μm. Data are representative of three independent experiments with three biological replicates (mean ± SD in panels A to C).