FIG 4.

Overexpressed TLR2, TLR3, TLR4, and TLR5 enhance IL-1β production during PRV infection. (A to D) HEK293T cells were transfected with the NF-κB reporter or a plasmid expressing TLR2, TLR3, TLR4, and TLR5 (200 ng/each). After transfection for 24 h, the cells were infected with PRV, and then luciferase assays were performed. The experiment shown is a representative experiment of three independent experiments with the mean ± SD of three technical replicates (one-way ANOVA) in panels A to D. **, 0.001 < P < 0.01; ***, P < 0.001. (E to H) HEK293T cells were transfected with plasmids expressing the indicated proteins. After transfection for 24 h, the cells were infected with PRV for 8 h and were assessed by Western blot analysis.