FIG 2.
Expression of the entire SARS-CoV-2 nsp1, but not its fragments, is cytotoxic. (A) The schematic representation of noncytopathic VEEV replicons used in this study and the structural elements of SARS-CoV-2 nsp1. (B) Equal amounts of the in vitro-synthesized replicons’ RNAs were electroporated into BHK-21 cells, and colonies of PurR cells were selected, as described in Materials and Methods. The means and SDs are presented. The statistical significance of differences was determined by one-way ANOVA with a post hoc Dunnett’s multiple-comparison test (n = 3). Only the statistically significant differences are indicated.