FIG 4.

HEK blebs-derived SLBs formed are mobile and retain available Sias. (a) Illustration of cell bleb-SLB formation. Chemically induced cell blebs are absorbed on a glass surface, then ruptured by fusogenic liposomes to form bilayers (created from Adobe illustrator). (b) The mobility of formed bleb-SLBs was determined by fluorescence recovery after photobleaching (FRAP). The images correspond to various times along the fluorescence recovery process of HEK293-SLB. (c) Left: Sias distribution on SLBs was determined by SNA lectin staining. SNA lectin, which recognizes α2-6 linked Sias, binds well to both Neu5Ac and Neu5Gc. Right: Sias incorporation was quantified by the number count of fluorescent particles in the field of view using ImageJ. Data were analyzed using PRISM software.