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. 2023 Mar 6;97(3):e00011-23. doi: 10.1128/jvi.00011-23

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FIG 3 — ORF8 interacts with Calnexin and HSPA5 via its immunoglobulin-like fold. (A) Illustration of the domains in WT and mutant ORF8. Rectangles represent the domains as indicated, and solid lines represent the deficient regions. (B-C) ΔSR binds to Calnexin (B) and HSPA5 (C): WT ORF8 (WT-Flag), two mutants (SR-Flag and ΔSR-Flag) or mock (Flag) were coexpressed with Calnexin-HA (B) or HSPA5-HA (C) in 293T cells, and immunoprecipitation products were analyzed by WB. (D–E) The ORF8-Calnexin (D) or ORF8-HSPA5 (E) interactions are independent of N78 glycosylation. WT ORF8 or N78Q (N78Q-Flag) was coexpressed with Calnexin-HA (D) or HSPA5-HA (E) in 293T cells, and immunoprecipitation products were analyzed by WB.