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. 2023 Mar 6;97(3):e00011-23. doi: 10.1128/jvi.00011-23

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FIG 4 — Specific ORF8-interacting domains of Calnexin and HSPA5. (A) Illustration of domains in WT and mutant Calnexin. (B–C) The ORF8 interaction with Calnexin is not exclusively dependent on a single domain. WT, single-domain-deficient Calnexin mutants (in B: ΔGlobular-HA, ΔArm-HA, ΔCtem-HA), or Calnexin mutants containing a single domain (in C: Globular-HA, Arm-HA, Ctem-HA) were coexpressed in 293T cells with ORF8-Flag, and immunoprecipitation products were analyzed by WB. (D) Protein docking of ORF8-Calnexin. Predicted interacting sites between Calnexin (gray) and ORF8 (green) are shown in yellow and red, respectively. (E) Illustration showing the domains in WT and mutant HSPA5. (F) HSPA5 interacts with ORF8 via both the NBD and SBD. Each of five single-domain-deficient HSPA5 mutants was coexpressed in 293T cells with ORF8-Flag, and immunoprecipitation products were analyzed by WB.