TABLE 2.
Primers used for real-time PCR analysis
| Gene name and primer | Primer sequencea |
|---|---|
| SADS-CoV N | |
| Forward | CTGACTGTTGTTGAGGTTA |
| Reverse | GACTGGCTAGAAGCTATG |
| Probe | FAM-TCACAGTCTCGTTCTCGCAATCA-TAMRA |
| IL-1β | |
| Forward | ATCTCGCAGCAGCACATCAA |
| Reverse | ACGGGAAAGACACAGGTAGC |
| IL-6 | |
| Forward | CACTTCACAAGTCGGAGGCT |
| Reverse | CTGCAAGTGCATCATCGTTGT |
| IL-8 | |
| Forward | ATCCTGATGCTCCATGGGTG |
| Reverse | GAAGCTTCATTGCCGGTGGA |
| GM-CSF | |
| Forward | GCTCACCCATCACTGTCACC |
| Reverse | GCGGGTCTGCACACATGTTA |
| TNF-α | |
| Forward | AGCCGATGGGTTGTACCTTG |
| Reverse | ATAGCAAATCGGCTGACGGT |
| CXCL10 | |
| Forward | GTCTGAGTGGGACTCAAGGG |
| Reverse | AGCTTCCCTATGGCCCTCAT |
| IFN-β | |
| Forward | CCAGCACTGGGTGGAATGAG |
| Reverse | AGTTGAGGACATCTCCCACG |
| IFN-γ | |
| Forward | CAGGCCATCAGCAACAACAT |
| Reverse | ACCTGTGGGTTGTTGACCTC |
| IFN-λ3 | |
| Forward | AGAACCCAAGCTGACCCTGT |
| Reverse | GCACCCTTGGCCTTTTTGAA |
| GAPDHb | |
| Forward | CTACCCCCAATGTGTCCGTC |
| Reverse | TGAAGTCGCAGGAGACAACC |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.
b
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.