FIG 6.

Megabat TRIM5α restricted MLV, but not HIV-1. (A) Protein expression of new megabat TRIM5α in stably expressing cells. TRIM5α proteins were detected with a specific antibody to the V5 tag, followed by the detection of α-tubulin on the stripped blot. (B) Titration curves of HIV-1 in U-2 OS and MLV in MDTF cells stably expressing rhesus and human TRIM5α (the top panels) and HeLa, CMS-C, and MDTF cells stably expressing megabat TRIM5α (the lower panels). Cells were infected in triplicate wells in three or four independent experiments, and all obtained data were averaged. Error bars represent the SD of results from independent experiments. Significant differences between the control (empty vector) and TRIM5α groups are indicated; P < 0.001 (***) is considered significant. Statistical significance was not detected by ANOVA in the lower panels. n.s., not significant. (C) Immunofluorescent staining of canine CMS-C cells stably expressing DemKT-1 and FBKT-1 TRIM5α. Higher magnification images (×63 objective lenses) of HeLa cells expressing megabat TRIM5α are shown. Green, TRIM5α; blue, DAPI (4′,6-diamidino-2-phenylindole).