TABLE 3.

PCR conditions used in this study^a

	PCR conditions
Viral RT product	Purpose	Temp (°C)	Duration	Cycles
Early and late RT product
Step 1	Initial denaturation	95	10 min	Hold
Step 2	Denaturation	95	15 s	45 cycles (steps 2–4)
Step 3	Annealing	55	30 s
Step 4	Extension	60	30 s
2-LTR circles
Step 1	Initial heating	50	2 min	Hold
Step 2	Initial denaturation	95	10 min	Hold
Step 3	Denaturation	95	15 s	45 cycles (steps 3 and 4)
Step 4	Annealing/extension	60	1 min
Proviruses
Step 1	Initial denaturation	95	10 min	Hold
Step 2	Denaturation	95	15 sec	45 cycles (steps 2–4)
Step 3	Annealing	60	1 min
Step 4	Extension	72	1 min

^aRT, reverse transcription; LTR, long terminal repeat.