FIG 7.

Characterization of VLP Envs from other HIV-1 strains. (A) 293T cells were transfected with pNL4-3.env infectious molecular clones expressing the NL4-3, JR-FL E168K and BG505 Envs. Seventy-two hours later, virus particles were purified and Env antigenicity on virus particles was analyzed as described in the Fig. 2C legend. Note that in A only, the JR-FL E168K mutant was used to allow recognition by V2 quaternary bNAbs (PG16, PGT145) (147, 148). The means and standard deviations of the results obtained in two independent experiments are reported in the bar graph at the right of the figure. The significance of the difference between AD8 Bam Env and other Envs was evaluated by a Student's t test; *, P < 0.05; **, P < 0.01; NA, not applicable. (B) The antigenicity of soluble gp120 versions of the indicated Envs was performed as described in the Fig. 4C legend. (C) The susceptibility of VLP Envs to gp120 shedding induced by BNM-III-170 or ice incubation was evaluated as described in the Fig. 5B and E legend. (D) Env glycoprotein expression, processing and incorporation into virus particles were examined as described in the Fig. 1A legend. The results are representative of those obtained in at least two independent experiments.