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SDS-PAGE analysis of IgG-depleted serum samples from three independent runs. The same amount of protein was loaded into each well and a commercial 1 μg antibody (Santa Cruz: sc-69879) was used as the standard for the IgG profiling. The IgG-depleted serum samples lacked the immunoglobulin heavy and light chains, while the eluted fractions contained both chains. Analysis of each lane showed a consistent banding pattern in the corresponding protein loadings.
