Table 1. Higher sfRNA:gRNA ratio in mosquito saliva increases DENV2 infection of human cells.

The experiment number, identity of the virus strain, concentration of gRNA and sfRNA, and sfRNA:gRNA ratio for pools of salivas collected from DENV2 infected mosquitoes are shown in the first five columns. The total volume of salivary inoculum (volume of infected saliva (inf.) + volume of uninfected saliva (uninf.)) used to infect Huh-7 cells and the gRNA copies added per well of Huh-7 cells are indicated in columns six and seven. The geometric mean, lower and upper 95% CI of dsRNA foci per cell at 2 days post infection are in column eight.

Exp.	DENV2 strain	gRNA (copies/ μl)	sfRNA (copies/ μl)	sfRNA: gRNA ratio	Total volume of saliva added per well of Huh-7 cells. (μl)	gRNA inoculum (copies/ well)	dsRNA foci per cell, geometric mean (lower and upper 95% CI)	p-value
1	PR6452	8 x 102	7.2 x 103	9	130 (130 inf. + 0 uninf.)	1 x 105	34.7 (26.2; 46.0)	0.01
	PR6452	9 x 102	4.2 x 104	46.7	130 (115 inf. + 15 uninf.)	1 x 105	64.1 (45.8; 89.8)
2	PR1940 (#1)	4.2 x103	2.9 x 103	6.7	185 (65.5 inf. + 119.5 uninf.)	2.8 x 105	48.5 (34.7; 67.9)	0.03
	PR1940 (#1)	1.5 x 103	4.6 x 104	30.2	185 (185 inf. + 0 uninf.)	2.8 x 105	78.8 (63.2; 98.3)
3	PR1940 (#2)	4 x 103	2.2 x 103	5.6	100 (65 inf. + 35 uninf.)	2.6 x 105	49.2 (23.6; 102.2)	0.27
	PR1940 (#2)	2.6 x 103	4.3 x 104	16.6	100 (100 inf. + 0 uninf.)	2.6 x 105	91.6 (53.2; 157.7)
4	PR9963	3.4 x 102	6.4 x 103	18.8	110 (83 inf. +27 uninf.)	2.8 x 104	51.0 (30.1; 86.5)	0.94
	PR9963	2.6 x 102	5.4 x 104	208	110 (110 inf. + 0 uninf.)	2.8 x 104	54.7 (33.8; 88.6)