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. 2023 Mar 17;14:1140703. doi: 10.3389/fphar.2023.1140703

FIGURE 1.

FIGURE 1

The expansion of T follicular helper cells during acute cardiac transplant rejection in mice (A). Illustration of the experimental design in (B–E). The murine intra-abdominal heterotopic cardiac transplantation model (BALB/c to B6 mice) was established, and the spleens of recipient mice were collected for flow detection at 0, 7, 14, and 21 days post-transplantation (B). The survival status of the grafts was observed every day through palpation. The survival time is illustrated in the survival curves (C). FCM analysis of the proportions of PD1+CXCR5+ Tfh and BCL6+CXCR5+ Tfh in the spleens of recipient mice at 0, 7, 14, and 21 days post-transplantation (D). The bar charts (n = 4) show the ratios and numbers of PD1+CXCR5+ Tfh cells in the spleens at different time points, as described in 1a (E). The bar charts (n = 4) show the ratios and numbers of BCL6+CXCR5+ Tfh cells in the spleens at different time points, as described in 1a.Error bars represent SD. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.