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. 2023 Mar 17;14:1140703. doi: 10.3389/fphar.2023.1140703

FIGURE 3.

FIGURE 3

Small-molecule BCL inhibitor FX1 does not protect acute cardiac transplant rejection in mice, but it inhibits T follicular helper cell expansion (A). Illustration of the experimental design in (B–E). The murine acute cardiac transplant model was established, and the mice were administered CTLA-4-Ig or the equivalent amount of PBS for 3 consecutive days. The survival time of the cardiac grafts was observed, and the spleens were collected for FCM analysis on the 14th postoperative day (B). The survival status of the grafts was observed every day through palpation, and the survival time is illustrated in the survival curves (n = 6) (C). Graft specimens were obtained on the 6th postoperative day, and they were stained with H&E to visualize pathological changes in the grafts. International Society for Heart and Lung Transplantation grading was performed on tissue sections of each group (n = 6) (D). The bar charts (n = 4) show the ratios and numbers of PD1+CXCR5+ Tfh and BCL6+CXCR5+ Tfh cells in the spleens on the 6th day post-transplantation. Error bars represent SD. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.