Fig. 1.

The downward static magnetic field alleviates alcohol-induced mouse liver damage. (A) Unmagnetized or magnetized NdFeB plates were used to provide sham, upward, or downward SMFs. Magnetic induction was measured at 1 cm above the plates with a magnet analyzer. (B) The whole mouse cage was placed on the magnetic plate, and the SMF on mice was ~0.1 to 0.2 T. (C) The mice were fed with unlimited access to the Lieber–DeCarli alcohol liquid diet containing either EtOH-fed or pair-fed with exposure to sham, upward, or downward SMFs for 3 weeks. (D) Food consumption and body weight were recorded every week. (E) Liver sections were subjected to H&E staining. (F) Liver section TUNEL assay and its quantification. Arrows indicate positively stained cells. n = 3 to 5 per group. (G) The protein levels of cleaved caspase 3, caspase 3, BCL2, and tubulin were detected by Western blot. All values represent means ± SEM. *P < 0.05 by Student's t test.