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. 2023 Mar 30;6:0097. doi: 10.34133/research.0097

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Fig. 3. — The downward SMF alleviates alcohol-induced inflammation and oxidative stress in liver. (A and B) Liver section immunohistochemical analysis of F4/80-positive cells and their quantification. (C) The mRNA expressions of proinflammation factors were detected by real-time fluorescence quantitative polymerase chain reaction, including IL-1β, IL-6, MCP, and TNFα-1. (D and E) Liver section immunofluorescent analysis of DHE-positive cells and their quantification. (F and G) Liver section immunohistochemical analysis of NRF2 and their quantification. (H) MDA in liver tissues was detected by the lipid peroxidation MDA assay kit. All values were expressed as means ± SEM. n = 3 to 5 per group. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student's t test.