Extended Data Fig. 1. T cell subsets across sites as measured by CyTOF.

(a) Scaled marker expression by T cells across sites as measured by CyTOF. Expression of the indicated surface and intracellular markers, as measured by CyTOF, used for unsupervised hierarchical clustering of T cells across tissue sites in Figure 1. Color intensity of individual cells denotes scaled expression of the indicated marker. (b) CyTOF analysis of T cells in circulation and in lymphoid and barrier sites shown in a t-SNE plot as in Fig. 1a stratified by individual donors. Arrows indicate clusters that differ between the younger and older donors. TEM, effector memory T cell; TEMRA, terminally-differentiated effector memory T cell.