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Efficient separation of EVs and soluble factors from CM. (A) Illustration of EVs and soluble factors isolation using SEC. (B) Particle number and protein concentration of individual fractions eluted from q-EV column. (C) Protein concentration of original CM and isolated CMdEV samples. (D) Transmission electron microscopy (TEM) of isolated EVs. Scale bar = 200 nm. (E) Western blotting assay of CD63 and CD9, and Calnexin in MSC cell lysate and EVs.
